LOCUS       sequence-79283-e         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Same as pRS316-RGR-GFP except for the HH ribozyme region which has a
            13 bp deletion at its 5' end, and for the HDV ribozyme region which 
            has a 15 bp deletion at its 3' end..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Gao Y, Zhao Y
  TITLE     Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro 
            and in vivo for CRISPR-mediated genome editing.
  JOURNAL   J Integr Plant Biol. 2013 Dec 30. doi: 10.1111/jipb.12152.
  PUBMED    24373158
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Integr 
            Plant Biol. 2013 Dec 30. doi: 10.1111/jipb.12152."
COMMENT     Sequence Label: pRS316-RGR-GFP-mm
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        108..509
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     misc_RNA        560..635
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 cgactcacta tagggcgaat tggagctcca ccgcggtggc ggccgctcta gaactagtgg
       61 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agcaacttct
      121 tttctttttt tttcttttct ctctcccccg ttgttgtctc accatatccg caatgacaaa
      181 aaaatgatgg aagacactaa aggaaaaaat taacgacaaa gacagcacca acagatgtcg
      241 ttgttccaga gctgatgagg ggtatctcga agcacacgaa actttttcct tccttcattc
      301 acgcacacta ctctctaatg agcaacggta tacggccttc cttccagtta cttgaatttg
      361 aaataaaaaa aagtttgctg tcttgctatc aagtataaat agacctgcaa ttattaatct
      421 tttgtttcct cgtcattgtt ctcgttccct ttcttccttg tttctttttc tgcacaatat
      481 ttcaagctat accaagcata caatcaactg tccgtgagga cgaaacgagt aagctcgtcc
      541 gtgctgaagt caagtttgag ttttagagct agaaatagca agttaaaata aggctagtcc
      601 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttg gccggcatgg tcccagcctc
      661 ctcgctgg
//
