LOCUS       sequence-79284-e         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express self-cleaving-ribozyme-flanked sgRNA cassette (RGR) 
            targeting GFP for CRISPR systems in yeast driven by ADH1 promoter. 
            RGR has a HH ribozyme at its 5', and an HDV ribozyme at its 3'..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Gao Y, Zhao Y
  TITLE     Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro 
            and in vivo for CRISPR-mediated genome editing.
  JOURNAL   J Integr Plant Biol. 2013 Dec 30. doi: 10.1111/jipb.12152.
  PUBMED    24373158
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Integr 
            Plant Biol. 2013 Dec 30. doi: 10.1111/jipb.12152."
COMMENT     Sequence Label: pRS316-RGR-GFP
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        110..511
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     ncRNA           518..554
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     misc_RNA        575..650
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     ncRNA           655..722
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     primer_bind     complement(914..938)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 tacgactcac tatagggcga attggagctc caccgcggtg gcggccgctc tagaactagt
       61 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagcaactt
      121 cttttctttt tttttctttt ctctctcccc cgttgttgtc tcaccatatc cgcaatgaca
      181 aaaaaatgat ggaagacact aaaggaaaaa attaacgaca aagacagcac caacagatgt
      241 cgttgttcca gagctgatga ggggtatctc gaagcacacg aaactttttc cttccttcat
      301 tcacgcacac tactctctaa tgagcaacgg tatacggcct tccttccagt tacttgaatt
      361 tgaaataaaa aaaagtttgc tgtcttgcta tcaagtataa atagacctgc aattattaat
      421 cttttgtttc ctcgtcattg ttctcgttcc ctttcttcct tgtttctttt tctgcacaat
      481 atttcaagct ataccaagca tacaatcaac tagcacgctg atgagtccgt gaggacgaaa
      541 cgagtaagct cgtccgtgct gaagtcaagt ttgagtttta gagctagaaa tagcaagtta
      601 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttggccgg
      661 catggtccca gcctcctcgc tggcgccggc tgggcaacat gcttcggcat ggcgaatggg
      721 acagctttgg acttcttcgc cagaggtttg gtcaagtctc caatcaaggt tgtcggcttg
      781 tctaccttgc cagaaattta cgaaaagatg gaaaagggtc aaatcgttgg tagatacgtt
      841 gttgacactt ctaaataagc gaatttctta tgatttatga tttttattat taaataagtt
      901 ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc
      961 tt
//
