LOCUS       sequence-79285-e         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Same as pRS316-RGR-GFP except for the HDV ribozyme region which has 
            a 15 bp deletion at its 3' end..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Gao Y, Zhao Y
  TITLE     Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro 
            and in vivo for CRISPR-mediated genome editing.
  JOURNAL   J Integr Plant Biol. 2013 Dec 30. doi: 10.1111/jipb.12152.
  PUBMED    24373158
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Integr 
            Plant Biol. 2013 Dec 30. doi: 10.1111/jipb.12152."
COMMENT     Sequence Label: pRS316-RGR-GFP-mHDV
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        109..510
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     ncRNA           517..553
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     misc_RNA        574..649
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(898..922)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 acgactcact atagggcgaa ttggagctcc accgcggtgg cggccgctct agaactagtg
       61 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagcaacttc
      121 ttttcttttt ttttcttttc tctctccccc gttgttgtct caccatatcc gcaatgacaa
      181 aaaaatgatg gaagacacta aaggaaaaaa ttaacgacaa agacagcacc aacagatgtc
      241 gttgttccag agctgatgag gggtatctcg aagcacacga aactttttcc ttccttcatt
      301 cacgcacact actctctaat gagcaacggt atacggcctt ccttccagtt acttgaattt
      361 gaaataaaaa aaagtttgct gtcttgctat caagtataaa tagacctgca attattaatc
      421 ttttgtttcc tcgtcattgt tctcgttccc tttcttcctt gtttcttttt ctgcacaata
      481 tttcaagcta taccaagcat acaatcaact agcacgctga tgagtccgtg aggacgaaac
      541 gagtaagctc gtccgtgctg aagtcaagtt tgagttttag agctagaaat agcaagttaa
      601 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttggccggc
      661 atggtcccag cctcctcgct ggcgccggct gggcaacatg cttcggagct ttggacttct
      721 tcgccagagg tttggtcaag tctccaatca aggttgtcgg cttgtctacc ttgccagaaa
      781 tttacgaaaa gatggaaaag ggtcaaatcg ttggtagata cgttgttgac acttctaaat
      841 aagcgaattt cttatgattt atgattttta ttattaaata agttataaaa aaaataagtg
      901 tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa attctt
//
