LOCUS       sequence-79288-e         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Guo X, Zhang T, Hu Z, Zhang Y, Shi Z, Wang Q, Cui Y, Wang F, Zhao H,
            Chen Y
  TITLE     Efficient RNA/Cas9-mediated genome editing in Xenopus tropicalis.
  JOURNAL   Development. 2014 Jan 8.
  PUBMED    24401372
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2014 Jan 8."
COMMENT     Sequence Label: pCS2-3xFLAG-NLS-SpCas9-NLS
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..96
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             103..123
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tttgcaggat cccatcgatt cgaattcatg gactataagg accacgacgg agactacaag
       61 gatcatgata ttgattacaa agacgatgac gataagatgg ccccaaagaa gaagcggaag
      121 gtcggtatcc acggagtccc agcagccgac aagaagtaca gcatcggcct ggacatcggc
      181 accaactctg tgggctgggc cgtgatcacc gacgagtaca aggtgcccag caagaaattc
      241 aaggtgctgg gcaacaccga ccggcacagc atcaagaaga acctgatcgg agccctgctg
      301 ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga gaaccgccag aagaagatac
      361 accagacgga agaaccggat ctgctatctg caagagatct tcagcaacga gatggccaag
      421 gtggacgaca gcttcttcca cagactggaa gagtccttcc tggtggaaga ggataagaag
      481 cacgagcggc accccatctt cggcaacatc gtggacgagg tggcctacca cgagaagtac
      541 cccaccatct accacctgag aaagaaactg gtggacagca ccgacaaggc cgacctgcgg
      601 ctgatctatc tggccctggc ccacatgatc aagttccggg gccacttcct gatcgagggc
      661 gacctgaacc ccgacaacag cgacgtggac aagctgttca tccagctggt gcagacctac
      721 aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg tggacgccaa ggccatcctg
      781 tctgccagac tgagcaagag cagacggctg gaaaatctga tcgcccagct gcccggcgag
      841 aagaagaatg gcctgttcgg caacctgatt gccctgagcc tgggcctgac ccccaacttc
      901 aagagcaact tcgacctggc cgaggatgcc aaactgcagc tgagcaagga cacctacgac
      961 gacgacctgg a
//
