LOCUS       sequence-79294-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector that contains an optimized S. pyogenes sgRNA 
            targeting the repetitive sequence of human MUC4 exon 3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Chen B, Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park 
            J, Blackburn EH, Weissman JS, Qi LS, Huang B
  TITLE     Dynamic Imaging of Genomic Loci in Living Human Cells by an 
            Optimized CRISPR/Cas System.
  JOURNAL   Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001.
  PUBMED    24360272
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.12"; journalName: "Cell"; date: "2013-12-19- 
            19"; volume: "155"; issue: "7"; pages: "1479-91"
COMMENT     Sequence Label: pSLQ1661-sgMUC4-E3(F+E)
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             119..299
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     808..824
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     809..825
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
       61 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgctg
      121 ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac
      181 aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt
      241 gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca gagagaccca
      301 gtacagtccg gatgcagctc tcgggccatg tgatgaaatg ctaggcggct gtcaaacctc
      361 cactctaata cttctctctc cgggtcatcc atcccatgca ggctcacagg gtgtaacaag
      421 cgggtgttct ctccttcatt ggcttcttct accttctctt gctcaactgg tactagcttg
      481 tagcaccatc caaaggtcag tggatatctg atccctggcc ctggtgtgta gttctgccaa
      541 tcagggaagt agccttgtgt gtggtagatc cacagatcaa ggatatcttg tcttcgttgg
      601 gagtgaatta gcccttccag tccccccttt tcttttaaaa agtggctaag atctacagct
      661 gccttgtaag tcattggtct taaaggtacc tgaggtgtga ctggaaaacc cacctcctcc
      721 tcctcttgtg cttctagcca ggcacaatca gcattggtag ctgctgtatt gctacttgtg
      781 attgctccat gtttttctag gtctcgatcg aggtcgacgg tatcgatgcg gggaggcggc
      841 ccaaagggag atccgactcg tctgagggcg aaggcgaaga cgcggaagag gcc
//
