LOCUS       sequence-79295-e         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector that contains an optimized S. pyogenes sgRNA 
            targeting the repetitive sequence of human MUC4 exon 3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Chen B, Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park 
            J, Blackburn EH, Weissman JS, Qi LS, Huang B
  TITLE     Dynamic Imaging of Genomic Loci in Living Human Cells by an 
            Optimized CRISPR/Cas System.
  JOURNAL   Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001.
  PUBMED    24360272
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.12"; journalName: "Cell"; date: "2013-12-19- 
            19"; volume: "155"; issue: "7"; pages: "1479-91"
COMMENT     Sequence Label: pSLQ1661-sgMUC4-E3(F+E)
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(282..315)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    371..959
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(424..444)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(842..853)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(962..978)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(963..979)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
       61 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
      121 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
      181 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
      241 catggacgag ctgtacaagt aagaattcgt cgagggacct aataacttcg tatagcatac
      301 attatacgaa gttatacatg tttaagggtt ccggttccac taggtacaat tcgatatcaa
      361 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
      421 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
      481 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
      541 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
      601 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
      661 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
      721 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
      781 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
      841 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
      901 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
      961 tcgataccgt cgacctcgat cgaga
//
