LOCUS       sequence-79300-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 
            849,865,877,907,980,1006 mutated to Ala .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Niewiadomski P, Kong JH, Ahrends R, Ma Y, Humke EW, Khan S, Teruel 
            MN, Novitch BG, Rohatgi R
  TITLE     Gli Protein Activity Is Controlled by Multisite Phosphorylation in 
            Vertebrate Hedgehog Signaling.
  JOURNAL   Cell Rep. 2013 Dec 24. pii: S2211-1247(13)00733-X. doi: 
            10.1016/j.celrep.2013.12.003.
  PUBMED    24373970
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2013 Dec 24. pii: S2211-1247(13)00733-X. doi: 
            10.1016/j.celrep.2013.12.003."
COMMENT     Sequence Label: pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    45..69
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             97..177
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             202..222
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gctcggatcc actagtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa
       61 aagcaggctg gcgccggaac caattcagtc gacatgtacc catacgatgt tccagattac
      121 gcttatcctt atgacgtacc tgactatgca tacccttatg atgtaccaga ctacgctggc
      181 gcggtaccaa gcggaggaag cgagaatctt tattttcagg gcactggagg aacgaattca
      241 aggatggagg cccaggccca cagctctacg gcgactgaga ggaagaaagc tgaaaattcc
      301 attgggaaat gtcccacgag aacagatgtc agcgagaagg ccgtggcctc tagtaccact
      361 tccaatgagg atgaaagtcc tggacagatc tatcaccgag agagaagaaa cgcaatcact
      421 atgcagcctc agagtgtgca gggtctcaac aaaatcagtg aggagccctc gacgtctagt
      481 gatgagaggg cctcgctgat caagaaagag atccatggct ctctaccaca tctggcggag
      541 ccctctctcc cttaccgtgg gactgtgttt gccatggatc cccggaatgg ctacatggag
      601 cctcactacc accctcctca tcttttccct gccttccatc ctcctgtacc aattgatgcc
      661 agacatcatg agggccgtta ccattatgat ccatctccta ttcctccatt acatgtgcct
      721 tctgccttat ctagtagccc gacgtatcca gacttgccct tcattaggat ctccccacac
      781 cgtaatccca ctgcagcctc agagtccccc ttcagccccc cacaccccta catcaa
//
