LOCUS       sequence-79305-e         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes N-3xHA-TEV-mouseGli2-C; amino acids 789,805,817,848 mutated 
            to Ala.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Niewiadomski P, Kong JH, Ahrends R, Ma Y, Humke EW, Khan S, Teruel 
            MN, Novitch BG, Rohatgi R
  TITLE     Gli Protein Activity Is Controlled by Multisite Phosphorylation in 
            Vertebrate Hedgehog Signaling.
  JOURNAL   Cell Rep. 2013 Dec 24. pii: S2211-1247(13)00733-X. doi: 
            10.1016/j.celrep.2013.12.003.
  PUBMED    24373970
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2013 Dec 24. pii: S2211-1247(13)00733-X. doi: 
            10.1016/j.celrep.2013.12.003."
COMMENT     Sequence Label: pEF5/FRT/DEST-3xHA/mGli2 P1-4A
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    45..69
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             97..177
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             202..222
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gctcggatcc actagtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa
       61 aagcaggctg gcgccggaac caattcagtc gacatgtacc catacgatgt tccagattac
      121 gcttatcctt atgacgtacc tgactatgca tacccttatg atgtaccaga ctacgctggc
      181 gcggtaccaa gcggaggaag cgagaatctt tattttcagg gcactggagg aacgagtcga
      241 caaatggaga cttctgcccc agcccctgca ctggagaaga aagaagccaa gagtggtctc
      301 ttggaggaca gcagcttccc cgacccaggg aaaaaggcct gtcctctggc ggtggccgca
      361 gctgtagccg cccacggagt tcctcagcag ctcctgccgg ctttccacgc gcctttgccg
      421 attgacatga gacaccagga gggaaggtac cattatgacc ctcactctgt ccacagtgta
      481 cacgggcctc ccaccctaag tggcagccct gtcatctcag atatctcctt gatacgactt
      541 tctccacacc ctgctggccc tggagagtca cccttcagcg cccaccaccc ctacgtgaac
      601 ccccatatgg agcactacct ccggtctgtg cacagcagcc ccacactctc aatgatctct
      661 gccgccaggg gcctcagccc tgctgatgtg gcccacgaac atctgaaaga gaggggactc
      721 tttagcctcg cagccccagg caccaaccct tcagactatt accaccagat gaccctcatg
      781 gcaagccacc ccacccctta tgggga
//
