LOCUS       sequence-79309-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 
            mutated to Ala.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Niewiadomski P, Kong JH, Ahrends R, Ma Y, Humke EW, Khan S, Teruel 
            MN, Novitch BG, Rohatgi R
  TITLE     Gli Protein Activity Is Controlled by Multisite Phosphorylation in 
            Vertebrate Hedgehog Signaling.
  JOURNAL   Cell Rep. 2013 Dec 24. pii: S2211-1247(13)00733-X. doi: 
            10.1016/j.celrep.2013.12.003.
  PUBMED    24373970
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2013 Dec 24. pii: S2211-1247(13)00733-X. doi: 
            10.1016/j.celrep.2013.12.003."
COMMENT     Sequence Label: pEF5/FRT/DEST-3xHA/mGli2 Pc-gA
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    47..71
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             99..179
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             204..224
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gagctcggat ccactagtcc agtgtggtgg aattctgcag atatcaacaa gtttgtacaa
       61 aaaagcaggc tggcgccgga accaattcag tcgacatgta cccatacgat gttccagatt
      121 acgcttatcc ttatgacgta cctgactatg cataccctta tgatgtacca gactacgctg
      181 gcgcggtacc aagcggagga agcgagaatc tttattttca gggcactgga ggaacgagtc
      241 gacaaatgga gacttctgcc ccagcccctg cactggagaa gaaagaagcc aagagtggtc
      301 tcttggagga cagcagcttc cccgacccag ggaaaaaggc ctgtcctctg gcggtggccg
      361 cagctgtagc cgcccacgga gttcctcagc agctcctgcc ggctttccac gcgcctttgc
      421 cgattgacat gagacaccag gagggaaggt accattatga ccctcactct gtccacagtg
      481 tacacgggcc tcccacccta agtggcagcc ctgtcatctc agatatctcc ttgatacgac
      541 tttctccaca ccctgctggc cctggagagt cacccttcag cgcccaccac ccctacgtga
      601 acccccatat ggagcactac ctccggtctg tgcacagcag ccccacactc tcaatgatct
      661 ctgccgccag gggcctcagc cctgctgatg tggcccacga acatctgaaa gagaggggac
      721 tctttagcct cgcagcccca ggcaccaacc cttcagacta ttaccaccag atgaccctca
      781 tggcaagcca ccccacccct tatggggacc ttctaatgca gagcgggggt gctgctagcg
      841 caccccatct ccatga
//
