LOCUS       sequence-79311-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes N-3xHA-TEV-mouseGli2-C; amino acids 
            216,220,224,230,248,789,805,817,848,923,956 mutated to Ala .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Niewiadomski P, Kong JH, Ahrends R, Ma Y, Humke EW, Khan S, Teruel 
            MN, Novitch BG, Rohatgi R
  TITLE     Gli Protein Activity Is Controlled by Multisite Phosphorylation in 
            Vertebrate Hedgehog Signaling.
  JOURNAL   Cell Rep. 2013 Dec 24. pii: S2211-1247(13)00733-X. doi: 
            10.1016/j.celrep.2013.12.003.
  PUBMED    24373970
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2013 Dec 24. pii: S2211-1247(13)00733-X. doi: 
            10.1016/j.celrep.2013.12.003."
COMMENT     Sequence Label: pEF5/FRT/DEST-3xHA/mGli2 Pc-gA/P1-6A
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    48..72
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             100..180
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             205..225
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 cgagctcgga tccactagtc cagtgtggtg gaattctgca gatatcaaca agtttgtaca
       61 aaaaagcagg ctggcgccgg aaccaattca gtcgacatgt acccatacga tgttccagat
      121 tacgcttatc cttatgacgt acctgactat gcataccctt atgatgtacc agactacgct
      181 ggcgcggtac caagcggagg aagcgagaat ctttattttc agggcactgg aggaacgagt
      241 cgacaaatgg agacttctgc cccagcccct gcactggaga agaaagaagc caagagtggt
      301 ctcttggagg acagcagctt ccccgaccca gggaaaaagg cctgtcctct ggcggtggcc
      361 gcagctgtag ccgcccacgg agttcctcag cagctcctgc cggctttcca cgcgcctttg
      421 ccgattgaca tgagacacca ggagggaagg taccattatg accctcactc tgtccacagt
      481 gtacacgggc ctcccaccct aagtggcagc cctgtcatct cagatatctc cttgatacga
      541 ctttctccac accctgctgg ccctggagag tcacccttca gcgcccacca cccctacgtg
      601 aacccccata tggagcacta cctccggtct gtgcacagca gccccacact ctcaatgatc
      661 tctgccgcca ggggcctcag ccctgctgat gtggcccacg aacatctgaa agagagggga
      721 ctctttagcc tcgcagcccc aggcaccaac ccttcagact attaccacca gatgaccctc
      781 atggcaagcc accccacccc ttatggggac cttctaatgc agagcggggg tgctgctagc
      841 gcaccccatc tccatga
//
