LOCUS       sequence-79314-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes N-3xHA-TEV-mouseGli3-Flag-C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Niewiadomski P, Kong JH, Ahrends R, Ma Y, Humke EW, Khan S, Teruel 
            MN, Novitch BG, Rohatgi R
  TITLE     Gli Protein Activity Is Controlled by Multisite Phosphorylation in 
            Vertebrate Hedgehog Signaling.
  JOURNAL   Cell Rep. 2013 Dec 24. pii: S2211-1247(13)00733-X. doi: 
            10.1016/j.celrep.2013.12.003.
  PUBMED    24373970
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2013 Dec 24. pii: S2211-1247(13)00733-X. doi: 
            10.1016/j.celrep.2013.12.003."
COMMENT     Sequence Label: pEF5/FRT/DEST-3xHA/mGli3/Flag
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    87..111
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(129..152)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cgagaccgag gagagggtta gggataggct taccttcgaa ccgcgggccc tctagactcg
       61 agcggccgcc actgtgctgg atatcaacca ctttgtacaa gaaagctggg tctagatatc
      121 tcgactcact tgtcatcgtc gtccttgtaa tctccgccag atcctggtgt tccgccacgc
      181 gtctgcataa ctgcaaggaa cttgctttct tctgcaaggg aggtcagcaa agaactcata
      241 tccccgatag ccatgttggt ggtgcccatg gatagggatg ggaatgggag ggatgcccgc
      301 ggagtggtga gacgggagga gctgtgggaa aggttctgaa taatacttgg gctcaaggcc
      361 cctgacatta ggctggtatg gtccccatca tctatgatgg catcaaaatc aatctgcaca
      421 ccttctaggt catgactctc aaagctgtca actgtgcttg ttacctggtt agcacctggg
      481 gagagtaact cagagttttc agtgccattc ccttggagca ggcagttggc atctgagagg
      541 gagaacgagc cagctttcct gtcttgctga ctgaagccca tggtttggtc atagaactga
      601 ccggaataat tctgcacggt agagcagagc tgctgggact gaccttgtgc ctccattttg
      661 atgctgttca ccctgctgct ctgactcatg tcactgagct gtccttgttg cagagtgagg
      721 ttgccccttg gcattgcctg acgcctgtta cccccatagc tggcacaggg ctggtagccc
      781 ctgatagctg ccatgcttga tggctggctg ctgttgtcct gagtccctgg taggcagctc
      841 tctgtccctt gatagatgtt gata
//
