LOCUS       sequence-79316-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 
            849,865,877,907,980,1006 mutated to Ala .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Niewiadomski P, Kong JH, Ahrends R, Ma Y, Humke EW, Khan S, Teruel 
            MN, Novitch BG, Rohatgi R
  TITLE     Gli Protein Activity Is Controlled by Multisite Phosphorylation in 
            Vertebrate Hedgehog Signaling.
  JOURNAL   Cell Rep. 2013 Dec 24. pii: S2211-1247(13)00733-X. doi: 
            10.1016/j.celrep.2013.12.003.
  PUBMED    24373970
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2013 Dec 24. pii: S2211-1247(13)00733-X. doi: 
            10.1016/j.celrep.2013.12.003."
COMMENT     Sequence Label: pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..47)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    100..124
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(142..165)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gtacgcgtag aatcgagacc gaggagaggg ttagggatag gcttaccttc gaaccgcggg
       61 ccctctagac tcgagcggcc gccactgtgc tggatatcaa ccactttgta caagaaagct
      121 gggtctagat atctcgactc acttgtcatc gtcgtccttg taatctccgc cagatcctgg
      181 tgttccgcca cgcgtctgca taactgcaag gaacttgctt tcttctgcaa gggaggtcag
      241 caaagaactc atatccccga tagccatgtt ggtggtgccc atggataggg atgggaatgg
      301 gagggatgcc cgcggagtgg tgagacggga ggagctgtgg gaaaggttct gaataatact
      361 tgggctcaag gcccctgaca ttaggctggt atggtcccca tcatctatga tggcatcaaa
      421 atcaatctgc acaccttcta ggtcatgact ctcaaagctg tcaactgtgc ttgttacctg
      481 gttagcacct ggggagagta actcagagtt ttcagtgcca ttcccttgga gcaggcagtt
      541 ggcatctgag agggagaacg agccagcttt cctgtcttgc tgactgaagc ccatggtttg
      601 gtcatagaac tgaccggaat aattctgcac ggtagagcag agctgctggg actgaccttg
      661 tgcctccatt ttgatgctgt tcaccctgct gctctgactc atgtcactga gctgtccttg
      721 ttgcagagtg aggttgcccc ttggcattgc ctgacgcctg ttacccccat agctggcaca
      781 gggctggtag cccctgatag ctgccatgct tgatggctgg ctgctgttgt cctgagtc
//
