LOCUS       sequence-79318-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 2ONU
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(621..639)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      635..682
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(857..862,863..879,880..885))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatgca tcatcatcat catcacagca
       61 gcggcagaga aaacttgtat ttccagggca ccagcctgac gcgtaaacaa tgcgatttta
      121 ctaagttaat tatggcgggc tatgacctgg aactgaacaa cggttctacc caggatttcg
      181 acgtgatgtt tcatgggcca aatggcacag cctacgaggg aggtatctgg aaagttcacg
      241 tcacgttgcc tgatgactat cccttcgcaa gtccgtcaat agggtttatg aataaacttc
      301 tccatccaaa cgtagatgaa gcttccggat cggtgtgttt agatgttatt aaccagacct
      361 ggactccgtt gtacagcctt gtcaatgtgt tcgaggtatt tctgccgcaa ctcctgacat
      421 atcctaatcc gtctgacccc ttaaacagtg atgcggcctc actgttgatg aaggacaaaa
      481 atatctacga ggagaaagtc aaggagtacg tgaaactgta cgcatccaaa gacctgtggg
      541 aatgatgacg aagcttgcgg ccgcactcga ggatccggct gctaacaaag cccgaaagga
      601 agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa
      661 acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc ccgcaagagg
      721 cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg
      781 atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac
      841 tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg
      901 aaga
//
