LOCUS       sequence-79319-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 2QG5
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatgca tcatcatcat catcacagca
       61 gcggcagaga aaacttgtat ttccagggca gcaccaaagg tgacatcaac caatattata
      121 ctttggaaaa tactatagga aggggttctt ggggtgaggt aaaaatagct gtccagaaag
      181 gaaccagaat tcgtagggca gcaaaaaaga ttccaaagta ctttgttgaa gatgttgatc
      241 gttttaaaca ggaaatcgaa attatgaagt ctttggatca tccaaacata attaggctat
      301 acgaaacttt tgaagataat actgatatat atttggttat ggaactatgt actggaggag
      361 agctttttga aagagttgta cacaagagag ttttcagaga aagcgatgct gcaagaatta
      421 tgaaagatgt tttaagcgct gtagcttatt gccataagtt aaatgtggct catagggatt
      481 tgaaacctga gaatttcctt ttcttgacag attctccaga ttctccctta aaattgattg
      541 actttggctt ggcagctaga ttcaagcctg gaaaaatgat gaggacaaag gttggaactc
      601 catattatgt gagcccgcag gttttggaag gtctttatgg cccagagtgt gatgaatggt
      661 cagcaggagt aatgatgtac gttttgcttt gtggctaccc tcctttcagt gcacctacag
      721 attctgaggt aatgcttaag atcagagaag gaacatttac atttccagaa aaagactggc
      781 ttaatgtttc tcctcaggca gaatctttaa taaggaggct gttgacaaag tctccaaaac
      841 aaagaattac ttctttgcaa gcattagaac atgagtggtt tgagaaacaa ctttcttcat
      901 ctcc
//
