LOCUS       sequence-7933-ex        9279 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9279)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 9279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG423GPD-ccdB-TAP
FEATURES             Location/Qualifiers
     source          1..9279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      68..1410
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    400..447
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        1437..1541
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1542..1610,1611..2402)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1760..1779)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2573..3161
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3062..3081
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3315..3332
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(3485..3490,3491..3508,3509..3515)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3523..3539
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3528..3550
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3547..3563
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3547..3563
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3582..3602
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3584..3602
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3631..4275
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     4169..4192
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     primer_bind     4281..4297
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4281..4297
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    4305..4429
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4454..4459,4460..4477,4478..4484)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4538..5197
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4604..4623)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5542..5847
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5726..5745
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5888..6012)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             6071..6148
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             6176..6196
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      6947..7194
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(6963..6981)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(7212..7231)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7213..7231)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7241..7258)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7241..7257)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7250..7272)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7402..7857
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7489..7508)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7699..7720
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8117..8776)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL
                     ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(8777..8963)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(9110..9129)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9229..9251
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagatgat ccaatatcaa aggaaatgat agcattgaag gatgagacta atccaattga
      121 ggagtggcag catatagaac agctaaaggg tagtgctgaa ggaagcatac gataccccgc
      181 atggaatggg ataatatcac aggaggtact agactacctt tcatcctaca taaatagacg
      241 catataagta cgcatttaag cataaacacg cactatgccg ttcttctcat gtatatatat
      301 atacaggcaa cacgcagata taggtgcgac gtgaacagtg agctgtatgt gcgcagctcg
      361 cgttgcattt tcggaagcgc tcgttttcgg aaacgctttg aagttcctat tccgaagttc
      421 ctattctcta gaaagtatag gaacttcaga gcgcttttga aaaccaaaag cgctctgaag
      481 acgcactttc aaaaaaccaa aaacgcaccg gactgtaacg agctactaaa atattgcgaa
      541 taccgcttcc acaaacattg ctcaaaagta tctctttgct atatatctct gtgctatatc
      601 cctatataac ctacccatcc acctttcgct ccttgaactt gcatctaaac tcgacctcta
      661 cattttttat gtttatctct agtattactc tttagacaaa aaaattgtag taagaactat
      721 tcatagagtg aatcgaaaac aatacgaaaa tgtaaacatt tcctatacgt agtatataga
      781 gacaaaatag aagaaaccgt tcataatttt ctgaccaatg aagaatcatc aacgctatca
      841 ctttctgttc acaaagtatg cgcaatccac atcggtatag aatataatcg gggatgcctt
      901 tatcttgaaa aaatgcaccc gcagcttcgc tagtaatcag taaacgcggg aagtggagtc
      961 aggctttttt tatggaagag aaaatagaca ccaaagtagc cttcttctaa ccttaacgga
     1021 cctacagtgc aaaaagttat caagagactg cattatagag cgcacaaagg agaaaaaaag
     1081 taatctaaga tgctttgtta gaaaaatagc gctctcggga tgcatttttg tagaacaaaa
     1141 aagaagtata gattctttgt tggtaaaata gcgctctcgc gttgcatttc tgttctgtaa
     1201 aaatgcagct cagattcttt gtttgaaaaa ttagcgctct cgcgttgcat ttttgtttta
     1261 caaaaatgaa gcacagattc ttcgttggta aaatagcgct ttcgcgttgc atttctgttc
     1321 tgtaaaaatg cagctcagat tctttgtttg aaaaattagc gctctcgcgt tgcatttttg
     1381 ttctacaaaa tgaagcacag atgcttcgtt caggtggcac ttttcgggga aatgtgcgcg
     1441 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     1501 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     1561 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     1621 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     1681 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     1741 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     1801 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     1861 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     1921 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     1981 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     2041 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     2101 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     2161 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     2221 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     2281 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     2341 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     2401 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     2461 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     2521 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     2581 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     2641 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     2701 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     2761 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     2821 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     2881 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     2941 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3001 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3061 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3121 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3181 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     3241 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     3301 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     3361 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     3421 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta cctcactcat taggcacccc
     3481 aggctttaca ctttatgctt ccggctccta tgttgtgtgg aattgtgagc ggataacaat
     3541 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     3601 gggaacaaaa gctggagctc ttcgagttta tcattatcaa tactcgccat ttcaaagaat
     3661 acgtaaataa ttaatagtag tgattttcct aactttattt agtcaaaaaa ttagcctttt
     3721 aattctgctg taacccgtac atgcccaaaa tagggggcgg gttacacaga atatataaca
     3781 ccgaaggtgc ctgggtgaac aggtttattc ctggcatcca ctaaatataa tggagcccgc
     3841 tttttaagct ggcatccaga aaaaaaaaga atcccagcac caaaatattg ttttcttcac
     3901 caaccatcag ttcataggtc cattctctta gcgcaactac agagaacagg ggcacaaaca
     3961 ggcaaaaaac gggcacaacc tcaatggagt gatgcaacct gcctggagta aatgatgaca
     4021 caaggcaatt gacccacgca tgtatctatc tcattttctt acaccttcta ttaccttctg
     4081 ctctctctga tttggaaaaa gctgaaaaaa aaggttgaaa ccagttccct gaaattattc
     4141 ccctacttga ctaataagta tataaagacg gtaggtattg attgtaattc tgtaaatcta
     4201 tttcttaaac ttcttaaatt ctacttttat agttagtctt ttttttagtt ttaaaacacc
     4261 agaacttagt ttcgacggat tctagaacta gtggatcccc catcacaagt ttgtacaaaa
     4321 aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata
     4381 aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg cggccgcatt
     4441 aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta
     4501 ggatccgtcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
     4561 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
     4621 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
     4681 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
     4741 tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt
     4801 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga
     4861 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg
     4921 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa
     4981 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc
     5041 ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc
     5101 gattcaggtt catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt
     5161 acaacagtac tgcgatgagt ggcagggcgg ggcgtaaacg ccgcgtggat ccggcttact
     5221 aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac
     5281 tgatatgtat acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg
     5341 acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc
     5401 tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg
     5461 aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg
     5521 acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt
     5581 tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg acggatggtg
     5641 atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg
     5701 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc
     5761 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
     5821 attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca
     5881 ggtcgaccat agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg
     5941 caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt cagctttctt
     6001 gtacaaagtg gtgatgggct gcaggaattc gatatcaagc ttatcgatag gtggaccagg
     6061 tggtggaatg aagcgacgat ggaaaaagaa tttcatagcc gtctcagcag ccaaccgctt
     6121 taagaaaatc tcatcctccg gggcacttga ttatggtacc ccaactactg cttctgaaaa
     6181 tctatatttt caaggtgaac taaaaactgc tgctttggct caacatgcga ttaagggtga
     6241 agctcaaaaa cttaatgact ctcaagctcc aaaagctgat gcgcaacaaa ataacttcaa
     6301 caaagatcaa caaagcgcct tctatgaaat cttgaacatg cctaacttaa acgaagcgca
     6361 acgtaacggc ttcattcaaa gtcttaaaga cgacccaagc caaagcacta acgttttagg
     6421 tgaagctaaa aaattaaacg aatctcaagc accgaaagct gataacaatt tcaacaaaga
     6481 acaacaaaat gctttctatg aaatcttgaa tatgcctaac ttaaacgaag aacaacgcaa
     6541 tggtttcatc caaagcttaa aagatgaccc aagccaaagt gctaacctat tgtcagaagc
     6601 taaaaagtta aatgaatctc aagcaccgaa agcggataac aaattcaaca aagaacaaca
     6661 aaatgctttc tatgaaatct tacatttacc taacttaaac gaagaacaac gcaatggttt
     6721 catccaaagc ctaaaagatg acccaagcca aagcgctaac cttttagcag aagctaaaaa
     6781 gctaaatgat gctcaagcac caaaagctga caacaaattc aacaaagaac aacaaaatgc
     6841 tttctatgaa attttacatt tacctaactt aactgaagaa caacgtaacg gcttcatcca
     6901 aagccttaaa gacgatcctt cggtgagcaa agaacagtca ctcgagtcat gtaattagtt
     6961 atgtcacgct tacattcacg ccctcccccc acatccgctc taaccgaaaa ggaaggagtt
     7021 agacaacctg aagtctaggt ccctatttat ttttttatag ttatgttagt attaagaacg
     7081 ttatttatat ttcaaatttt tctttttttt ctgtacagac gcgtgtacgc atgtaacatt
     7141 atactgaaaa ccttgcttga gaaggttttg ggacgctcga aggctttaat ttgcggccgg
     7201 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     7261 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     7321 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     7381 gcctgaatgg cgaatggcgc gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     7441 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     7501 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     7561 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     7621 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     7681 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     7741 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     7801 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcct
     7861 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatag atccgtcgag
     7921 ttcaagagaa aaaaaaagaa aaagcaaaaa gaaaaaagga aagcgcgcct cgttcagaat
     7981 gacacgtata gaatgatgca ttaccttgtc atcttcagta tcatactgtt cgtatacata
     8041 cttactgaca ttcataggta tacatatata cacatgtata tatatcgtat gctgcagctt
     8101 taaataatcg gtgtcactac ataagaacac ctttggtgga gggaacatcg ttggtaccat
     8161 tgggcgaggt ggcttctctt atggcaaccg caagagcctt gaacgcactc tcactacggt
     8221 gatgatcatt cttgcctcgc agacaatcaa cgtggagggt aattctgcta gcctctgcaa
     8281 agctttcaag aaaatgcggg atcatctcgc aagagagatc tcctactttc tccctttgca
     8341 aaccaagttc gacaactgcg tacggcctgt tcgaaagatc taccaccgct ctggaaagtg
     8401 cctcatccaa aggcgcaaat cctgatccaa acctttttac tccacgcgcc agtagggcct
     8461 ctttaaaagc ttgaccgaga gcaatcccgc agtcttcagt ggtgtgatgg tcgtctatgt
     8521 gtaagtcacc aatgcactca acgattagcg accagccgga atgcttggcc agagcatgta
     8581 tcatatggtc cagaaaccct atacctgtgt ggacgttaat cacttgcgat tgtgtggcct
     8641 gttctgctac tgcttctgcc tctttttctg ggaagatcga gtgctctatc gctaggggac
     8701 caccctttaa agagatcgca atctgaatct tggtttcatt tgtaatacgc tttactaggg
     8761 ctttctgctc tgtcatcttt gccttcgttt atcttgcctg ctcatttttt agtatattct
     8821 tcgaagaaat cacattactt tatataatgt ataattcatt atgtgataat gccaatcgct
     8881 aagaaaaaaa aagagtcatc cgctagggga aaaaaaaaaa tgaaaatcat taccgaggca
     8941 taaaaaaata tagagtgtac tagaggaggc caagagtaat agaaaaagaa aattgcggga
     9001 aaggactgtg ttatgacttc cctgactaat gccgtgttca aacgatacct ggcagtgact
     9061 cctagcgctc accaagctct taaaacggga atttatggtg cactctcagt acaatctgct
     9121 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     9181 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     9241 tgtgtcagag gttttcaccg tcatcaccga aacgcgcga
//
