LOCUS       sequence-79343-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4IFG
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatgca tcatcatcat catcacagca
       61 gcggcagaga aaacttgtat ttccagggca tggggcagca ggaaagcact cttgggggtg
      121 cggccgggga gcctcgctcg cgcggtcatg cggcggggac cagcggtgga ccaggagacc
      181 atctccacgc gacgcccggg atgttcgttc agcattcgac tgcgatcttc tccgaccggt
      241 acaagggaca gcgggtgttg gggaaggggt cttttggtga ggtgattctg tgcaaagaca
      301 agatcaccgg tcaggagtgt gcggtgaagg tcattagcaa gcgccaagtg aagcagaaga
      361 cggacaagga gtctctgctt cgcgaggtgc agttgctgaa gcagctggac caccccaaca
      421 tcatgaagct gtatgaattc ttcgaggaca aaggctactt ctacctcgtc ggcgaagtgt
      481 acacgggagg cgagttgttc gacgagatca tttcccgcaa gcgcttcagc gaagtcgatg
      541 cggcgcggat catccgccaa gtcctcagcg gcatcacgta catgcacaaa aataaaatcg
      601 tacatcggga cctcaaaccg gaaaacctgc tcctggaaag caaaagcaag gacgcgaaca
      661 tccgcatcat cgactttggc ctcagcactc acttcgaagc gagcaagaag atgaaggaca
      721 aaatcggaac tgcatactac atcgcacccg aggtcctcca cggcacttac gacgagaaat
      781 gcgacgtatg gtcgaccggt gttattctct acatccttct ttccggatgc ccacccttca
      841 atggagcaaa cgagtacgac atcctgaaga a
//
