LOCUS       sequence-79345-e         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 3EOE
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatgca tcatcatcat catcacagca
       61 gcggcagaga aaacttgtat ttccagggca tggcgagcaa acagccgcaa accctgtcgg
      121 ctggcgcagt ggaaagtggt cgtgttgctc gcctggtatc agcttcttcc gtcatgacgc
      181 agcaactggg gaaatcgact aatattcgta tgagccagat cctggagcca cgcagtgaag
      241 aggattggac agcccatcgt acccgcattg tgtgcacgat gggacctgca tgttggaacg
      301 ttgacactct ggtcaaaatg atcgatgctg gcatgaatgt atgccgtctg aacttttcac
      361 acggtgacca tgaaacacac gcgcgcaccg tgcagaatat tcaagaagcc atgaaacagc
      421 gtccggaggc acgcctggct atcctgctgg atacgaaagg gccagaaatt cgtaccggat
      481 tcctgaaaga ccataaacct atcactctgc aacagggcgc gacactgaaa attgttacgg
      541 attataacct gatcggtgat gagacaacta ttgcctgttc ctacggcgca ctgccgcagt
      601 cggtcaaacc gggtaataca atcctgattg ctgacgggag cctgagtgtt aaagtagttg
      661 aagtgggaag tgattatgtc atcacccaag cgcagaacac ggccaccatt ggcgaacgca
      721 aaaatatgaa cctgccaaat gttaaagtac aactgcctgt gatcggtgag aaagacaaac
      781 acgatattct gaactttggc attccgatgg gttgcaattt catcgcagcg tcatttgtcc
      841 agtccgctga cgatgttcgt tacattcgcg ggctgctggg accgcgtggc cgccatatcc
      901 gtattatccc aaaa
//
