LOCUS       sequence-79346-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4IJ8
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             88..108
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             678..695
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(748..766)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      762..809
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgt cgtactacca tcaccatcac
       61 catcacgatt acgatatccc aacgaccgaa aacctgtatt ttcagggcgc catgggatcc
      121 aggaagagca aagccgagct gcagtctgaa gaaaggaaaa gaatagatga attgattgaa
      181 agtgggaagg aagaaggaat gaagattgac ctcatcgatg gcaaaggcag gggtgtgatt
      241 gccaccaagc agttctcccg gggtgacttt gtggtggaat accacgggga cctcatcgag
      301 atcaccgacg ccaagaaacg ggaggctctg tacgcacagg acccttccac gggctgctac
      361 atgtactatt ttcagtatct gagcaaaacc tactgcgtgg atgcaactag agagacaaat
      421 cgcctaggaa gactgatcaa tcacagcaaa agtgggaact gccaaaccaa actgcacgac
      481 atcgacggcg tacctcacct catcctcatc gcctcccgag acatcgcggc tggggaggag
      541 ctcctgtatg actatgggga ccgcagcaag gcttccattg aagcccaccc gtggctgaag
      601 cattaagaat tcaaaggcct acgtcgacga gctcactagt cgcggccgct ttcgaatcta
      661 gagcctgcag tctcgagcac caccaccacc accactgaga tccggctgct aacaaagccc
      721 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg
      781 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga
      841 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
      901 gaccgctaca cttgccagcg ccctagcgcc
//
