LOCUS       sequence-79349-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses the codon optimized ORF1 of L1RP with his tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Wagstaff BJ, Kroutter EN, Derbes RS, Belancio VP, Roy-Engel AM
  TITLE     Molecular reconstruction of extinct LINE-1 elements and their 
            interaction with nonautonomous elements.
  JOURNAL   Mol Biol Evol. 2013 Jan;30(1):88-99. doi: 10.1093/molbev/mss202. 
            Epub 2012 Aug 23.
  PUBMED    22918960
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/molbev/mss202"; journalName: "Mol Biol Evol"; date: 
            "2013-01"; volume: "30"; issue: "1"; pages: "88-99"
COMMENT     Sequence Label: pBudORF1-CH
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..27)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gaattctcaa tggtgatggt gatgatgcat cttggcgtgg ttttgcaggg gctgataccg
       61 gttgttgcgc tccatgttca gggcctcctt cagcagctcc ttcagggcgg ggcgggtggt
      121 cacgaagtcg cgcagcatct gcttgtcgat gaagtatttg atctcgccct cgctgatgaa
      181 gctcagcttg gcggggtagc tgatgcgggg ctggaagttc ttctccttca ggatgttgaa
      241 gattggcccc cactcgcggc gggcctgtag ggtctcggcg ctcaggtcgg cggtcaggcg
      301 gatgggcttg cccttcaggg taacccggcc cttctcgcgg gcggcgcgca gcatcttctc
      361 cttcatctcc accttggtga agcgcacgat gatgtggcgg ggggtggcgc ggcggctgct
      421 gtagcgctgg ggggtgcgct ggatctcctg gatctgcacg ttggcctggc gggccaggtt
      481 ggggaagttc tcctggatga tgtcctgtag ggtgttctcc agcttggtgc cgttctccac
      541 gtcgctctcg ggcacgccga tcaggcgcag gttggggcgc ttcacgcagt cccatatctc
      601 ctggaggctc tgctcgttgc gcttgatgcg cttctcgcgg aacttgccct cgcgcttcat
      661 ctcgttcatc tcgtcctcca ttgcgctcac gcgctcctcc agctggtcgc agcggctgcg
      721 caggctgcgg cactcctcgc gcagctcgcg ggccttggtc ttcagctcca tcagctcctt
      781 caggcacttc tcggtgttgg tgatgcgggt gatgcactcc tccaggttct tctcgaagtt
      841 ctccacctcc ttgcccttgg tctggatgtc ctcgcgcagc tcgctgtagt t
//
