LOCUS       sequence-79351-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses U6 RNA, using its own pol III enhancer sequence and tagged
            with the self splicing intron neo cassette .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Kroutter EN, Belancio VP, Wagstaff BJ, Roy-Engel AM
  TITLE     The RNA polymerase dictates ORF1 requirement and timing of LINE and 
            SINE retrotransposition.
  JOURNAL   PLoS Genet. 2009 Apr;5(4):e1000458. doi: 
            10.1371/journal.pgen.1000458. Epub 2009 Apr 24.
  PUBMED    19390602
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2009-04"; volume: "5(4):e1000458. doi"; pages: " 
            10.1371/journal.pgen.1000458. Epub 2009 Apr 24"
COMMENT     Sequence Label: pU6-neo-TET
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(263..282)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 tttatatatc ttgtggaaag gacgaaacac cgtgctcgct tcggcagcac atatactaaa
       61 attggaacga tacagagaag attagcatgg cccctgcgca aggatgacac gcaaattcgt
      121 gaagcgttcc atattgacgt ctcaaatccc ctcagaagaa ctcgtcaaga aggcgataga
      181 aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc
      241 attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt
      301 ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattc tccaccatga
      361 tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg
      421 ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat
      481 cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt
      541 ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca
      601 tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt
      661 cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag
      721 gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctgtaat tagcaatata
      781 atgaattgca gggaaaagtt atcaggcatg cacctggtag ctagtcttta aaccaataga
      841 ttgcatcggt ttaaaaggca agaccgtcaa attg
//
