LOCUS       sequence-79352-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 2QG7
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcagagaa aacttgtatt tccagggcaa gcaagccaag tacctcataa
      121 gactttatgg acccaaaacg gacgaaatta ttaacaggga aagagaaaaa aaaatctcct
      181 gcattttata taataaaaat atagccaaaa aaatatacgt tttttttacc aacgggagaa
      241 ttgaggaatt catggatggc tatgctcttt ccagggagga tataaaaaac ccaaagttcc
      301 agaaactcat tgccaagaat ctgaagctcc tacatgatat caagttgaat gaaaatttgt
      361 ataaagagct tcaagtgact cagaaggttc ctgggaccag accgtccttc ctgtggaata
      421 ccatctggaa atactttcat ctgctgaatg aagagcggaa gaagatctgc tccttcgatg
      481 caaaggcgaa cattttgaag ctcatcgatt ttgacgtcct gcgcgacagc attgttgagg
      541 tggagagcct ctgcaagagg gagaactccc ccattgtcct gtgccattgc gatttgctct
      601 cctccaacat tattaacacc gtgggggggg gagaagcagg cgaattaggc gaagcgggag
      661 aaacgggcga agggggagaa acaggcgaag ggggagaaac aggcgaaggg ggagaaacag
      721 gcgaaggggg cgaaggggac agcatctcct tcatcgattt cgagtactcc tgccccatgg
      781 agcgcgccta cgacatcgcc aaccacttta acgagtacgc gggattcaac tgcgactggg
      841 acttaactcc ct
//
