LOCUS       sequence-79353-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 3CLV
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(789..807)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      803..850
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatgca tcatcatcat catcacagca
       61 gcggcagaga aaacttgtat ttccagggca tggaaaagaa aagtagttat aaaacagttt
      121 tattaggaga atcatcagta ggaaaatcaa gtatagtttt acgattaaca aaagatactt
      181 ttcatgagaa tactaacaca acaataggtg catctttttg tacatatgta gtaaatttaa
      241 atgatataaa tataaagaat aatagtaata atgaaaaaaa taataatata aattcaataa
      301 atgatgataa taatgttatt ataacaaatc aacataataa ttataatgaa aacttatgta
      361 atataaaatt tgatatatgg gatacagctg gacaagaaag atatgcaagt attgtcccac
      421 tatattatag aggtgctact tgtgctatag tagtttttga tattagcaat tcaaatactt
      481 tagatcgagc taagacatgg gttaatcaat taaaaattag tagtaactat attattattt
      541 tagttgctaa taaaatagat aaaaacaaat tccaagttga tatattagaa gtacaaaaat
      601 atgctcaaga caataattta ctttttattc aaacaagtgc caaaactgga acaaacataa
      661 aaaatatatt ctacatgctt gctgaagaaa tttataaaaa tattataaat tgatgacgaa
      721 gcttgcggcc gcactcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc
      781 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
      841 gggttttttg ctgaaaggag gaactatatc cggatatccc gcaagaggcc cggcagtacc
      901 ggcataacca agcctatgcc tacagcatcc aggg
//
