LOCUS       sequence-79354-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 3EOZ
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(756..774)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      770..817
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatgca tcatcatcat catcacagca
       61 gcggcagaga aaacttgtat ttccagggca atacaacgaa acatattata ttagttagac
      121 atggacaata tgaaagaaga tataaagatg atgagaattc taaacgttta actaaagaag
      181 ggtgtaaaca agctgatata acgggtaaaa aattaaaaga tattttaaat aataaaaagg
      241 ttagtgttat ttatcattca gatatgataa gagctaaaga aacggctaat attataagta
      301 aatattttcc tgatgctaat ttaataaatg atccaaattt aaatgaagga accccttatt
      361 tacctgaccc tcttccaaga cattcaaaat ttgatgctca aaaaattaaa gaagataata
      421 aaagaataaa taaagcttat gaaacttatt tttataaacc tagtggtgat gaagatgaat
      481 atcaattagt aatatgtcac ggaaatgtaa ttagatattt cttgtgtaga gctctacaaa
      541 ttcctttatt tgcatggtta cgattttcaa gttataattg tggtattaca tggttagttt
      601 tagatgatga gggttctgta gttttaagag aatttggttc cgtttctcac ctcccctttg
      661 aaagtgtaac atatttttga tgacgaagct tgcggccgca ctcgaggatc cggctgctaa
      721 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
      781 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg
      841 atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg
      901 gtgacggtgc cgaggatgac
//
