LOCUS       sequence-79355-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 3B7P
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcagagaa aacttgtatt tccagggcag caaaaaatgg ttctccgaat
      121 tttcgatcat gtggccgggg caagccttca gcctgaagat caaaaaaatc ctgtacgaga
      181 ccaagtccaa ataccagaac gtcctggtgt tcgagtcgac gacctacggc aaagtcttgg
      241 tgcttgacgg agtaattcag ctgactgaaa aggacgagtt tgcataccac gagatgatga
      301 cgcatgtccc aatgacagtt agcaaagagc cgaaaaacgt gctcgtagtt ggtgggggag
      361 atggcggtat aattcgtgaa ttatgcaagt ataaatccgt ggagaacatc gacatctgcg
      421 agattgacga gactgtcatt gaggtgagta aaatctactt caagaacatc agctgcggct
      481 acgaggacaa acgcgtcaat gtgttcatcg aggacgcttc aaaattcctg gagaacgtca
      541 ccaatacgta cgacgtgatt atcgtggact cgtctgaccc tattggtccc gcggaaacat
      601 tgtttaatca gaatttctac gagaagatct acaacgccct gaaaccgaac gggtattgcg
      661 tcgcacaatg tgaaagcctt tggattcacg tgggaaccat aaaaaacatg atcggctacg
      721 ctaagaaact gttcaaaaag gtcgagtacg cgaacatctc catcccgacg tacccgtgtg
      781 gttgtattgg cattctgtgt tgcagtaaaa ccgacaccgg tctcactaaa ccgaataaga
      841 aactggagtc gaaagagttc gccgatttaa agtactacaa ctac
//
