LOCUS       sequence-79358-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 2PWQ
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(762..780)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      776..823
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcagagaa aacttgtatt tccagggctc caaagaactg ttacgcctgc
      121 agaaggagtt gaaagatatt gagaatgaga acgtgcaaga gatcgacgcg catatcaaag
      181 actcgaactt tttcgagtgg gttggcttca tcaagggtcc ggaggggacc ccatatgaag
      241 gaggccactt tacgcttgcc attactatac ctaatgatta cccgtataat ccgcccaaaa
      301 ttaaattcgt caccaagatc tggcacccga acattagctc ccagacaggt gcaatatgtc
      361 tggatgtgct caaaaacgag tggtctccgg ctttaacgat tcgtactgcg ttgcttagta
      421 ttcaggccct gctctcagac ccacaacctg atgatcccca ggacgcagaa gtagctaaaa
      481 tgtacaagga aaatcatgcg ctgtttgtta aaacagcctc ggtctggacc aaaacgtttg
      541 caaccgggcc aaaggaagaa cctcgggaag taatcataaa aaaaatcact gagatgggct
      601 tcagcgaaga tcaagctaag aatgcgttaa tcaaagccaa ctggaacgag acgctggcat
      661 tgaataccct tctggaaaat tcctgatgac gaagcttgcg gccgcactcg aggatccggc
      721 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
      781 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
      841 atccggatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca
      901 tcc
//
