LOCUS       sequence-79360-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 2PMY
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             338..355
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(408..426)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      422..469
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      506..864
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(593..612)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     803..824
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatgca tcatcatcat catcacagca
       61 gcggcagaga aaacttgtat ttccagggcg cggatgggga cggagaggag ctggcccggc
      121 tgcgctcagt cttcgccgcc tgcgacgcga accgctcggg gcgcctggag cgcgaggagt
      181 tccgggcact gtgcacggag ctgcgggtgc ggccggccga cgccgaggca gtattccagc
      241 ggctggacgc cgaccgtgac ggcgccatca ccttccagga gttcgcgcgt ggcttcctcg
      301 ggtccctctg atgacgaagc ttgcggccgc actcgagcac caccaccacc accactgaga
      361 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
      421 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
      481 aactatatcc ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
      541 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
      601 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
      661 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
      721 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
      781 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
      841 atctcggtct attcttttga tttataa
//
