LOCUS       sequence-79361-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 2LV9
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             359..376
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(429..447)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      443..490
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      527..885
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(614..633)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     824..845
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatgca tcatcatcat catcacagca
       61 gcggcagaga aaacttgtat ttccagggct ctgaggatgg aagttatggt actgatgtaa
      121 ccaggtgcat atgtggtttt acacatgatg atggatacat gatctgttgt gacaaatgca
      181 gcgtttggca acatattgac tgcatgggga ttgataggca gcatattcct gatacatatc
      241 tatgtgaacg ttgtcagcct aggaatttgg ataaagagag ggcagtgcta ctacaacgcc
      301 ggaaaaggga aaatatgtca gatggtgatt gatgacgaag cttgcggccg cactcgagca
      361 ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc
      421 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
      481 gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc
      541 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
      601 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
      661 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
      721 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
      781 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
      841 actggaacaa cactcaaccc tatctcggtc tattcttttg attta
//
