LOCUS       sequence-79362-e         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 3CBQ
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             650..667
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(720..738)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      734..781
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(905..924)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatgca tcatcatcat catcacagca
       61 gcggcagaga aaacttgtat ttccagggcc aaaaggatgg catcttcaag gtcatgctag
      121 tgggggagag cggcgtgggc aagagcaccc tagcaggcac ttttggtggt ctccagggag
      181 acagtgctca cgaaccggag aacccagagg atacctatga gagacgcatc atggtggata
      241 aggaggaagt gactctagtc gtttatgaca tctgggaaca gggggatgca ggagggtggc
      301 tgcgggacca ctgccttcag accggggacg cctttctcat cgtcttctca gtcaccgacc
      361 gacggagttt ctccaaagtt ccagagaccc tacttcggct ccgggctggg aggccgcacc
      421 acgacctacc cgttatcctc gttggaaaca agagcgactt ggcccgctcc cgggaggtat
      481 cactggagga gggccgccac ctggccggga cgctgagctg caagcacatc gagacgtcgg
      541 ccgcactgca ccacaacacg agggagctct tcgagggcgc ggtgcgccag atccggctgc
      601 ggcggggccg aaaccacgcc tgatgacgaa gcttgcggcc gcactcgagc accaccacca
      661 ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
      721 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
      781 gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta
      841 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
      901 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
      961 gctctaaatc gggg
//
