LOCUS       sequence-79364-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4IJD
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             782..799
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(852..870)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatgca tcatcatcat catcacagca
       61 gcggcagaga aaacttgtat ttccagggca gcgagccgca ggatgatgat tacctctatt
      121 gtgagatgtg tcagaacttc ttcattgaca gctgtgctgc ccatgggccc cctacatttg
      181 taaaggacag tgcagtggac aaggggcacc ccaaccgttc agccctcagt ctgcccccag
      241 ggctgagaat tgggccatca ggcatccctc aggctgggct tggagtatgg aatgaggcat
      301 ctgatctgcc gctgggtctg cactttggcc cttatgaggg ccgaattaca gaagacgaag
      361 aggcagccaa caatggatac tcctggctga tcaccaaggg gagaaactgc tatgagtatg
      421 tggatggaaa agataaatcc tgggccaact ggatgaggta tgtgaactgt gcccgggatg
      481 atgaagagca gaacctggtg gccttccagt accacaggca gatcttctat agaacctgcc
      541 gagtcattag gccaggctgt gaactgctgg tctggtatgg ggatgaatac ggccaggaac
      601 tgggcatcaa gtggggcagc aagtggaaga aagagctcat ggcagggaga gaaccaaagc
      661 cagagatcca tccatgtccc tcatgctgtc tggccttttc aagtcagaaa tttctcagtc
      721 aacatgtaga acgcaatcac tcctctcaga actgatgacg aagcttgcgg ccgcactcga
      781 gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt
      841 ggctgctgcc accgctgagc aataactagc ataacccctt gggg
//
