LOCUS       sequence-79365-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 2R2O
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             65..85
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             475..492
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(545..563)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      559..606
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(730..749)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgcatcat catcatcatc acagcagcgg
       61 cagagaaaac ttgtatttcc agggcgatgt ggagtaccgt cccctgacct tgaatgcact
      121 attggctgtg gggcctgggg caggagaggc ccagggcgtg cccgtgaagg tcctagactg
      181 tgacaccatc tcccaggcaa aggagaagat gctggaccag ctttataaag gagtgcctct
      241 cacccagcgg ccagaccctc gcacccttga tgttgagtgg cggtctgggg tggccgggca
      301 cctcattctt tctgacgagg atgtcacttc tgaggtccag ggtctgtgga ggcgcctgaa
      361 cacactgcag cattacaagg tcccagatgg agcaactgtg gccctcgtcc cctgcctcac
      421 caagcatgtg ctccgggaaa accagtgatg acgaagcttg cggccgcact cgagcaccac
      481 caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
      541 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
      601 tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg
      661 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
      721 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
      781 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
      841 accccaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gataga
//
