LOCUS       sequence-79366-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 2Q3F
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             659..676
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(729..747)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      743..790
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatgca tcatcatcat catcacagca
       61 gcggcagaga aaacttgtat ttccagggcg aaaaaattca cctcagcttt tatctgacaa
      121 gcatatatga tcattcaata tttgaagctt ttagcaaagt tgttcagaaa ctgattccac
      181 aactcccaac tctggagaat ttgctgaaca tctttatctc aaattctgga attgaaaagg
      241 catttctatt tgatgtggtc agtaaaattt atattgcaac tgatagtact ccggtggata
      301 tgcaaaccta tgagctctgc tgtgatatga tagatgtggt tattgacatc tcttgtattt
      361 atggtctcaa agaagatgga gcaggaaccc cctatgacaa ggaatccaca gccatcataa
      421 agcttaataa tacaaccgtg ctttatttaa aagaggtgac aaagttcctg gctctcgttt
      481 gctttgtcag agaggaaagc tttgaaagaa aagggctaat tgactataat tttcattgct
      541 tccggaaggc cattcatgaa gtttttgagg tgagaatgaa agtagtaaaa tctcgaaagg
      601 ttcagaatcg gctgcagaag aaaaagagat gatgacgaag cttgcggccg cactcgagca
      661 ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc
      721 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
      781 gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc
      841 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttg
//
