LOCUS       sequence-79368-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 3BPJ
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             554..571
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(624..642)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      638..685
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(809..828)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatgca tcatcatcat catcacagca
       61 gcggcagaga aaacttgtat ttccagggca aaatagcaga gaagataaaa gagaaagaac
      121 ggcaacagaa gaaaaggcaa gaagaaatta aaaagaggtt agaagaaccc gaagaaccta
      181 aagtgctaac accagaagaa caattagcag ataaactgcg gctaaagaaa ttacaggaag
      241 agtcagacct cgaattagca aaggaaactt ttggtgttaa taatgcagtt tatggaatag
      301 atgctatgaa cccatcttca agagatgact ttacagagtt tggaaagtta ctaaaagata
      361 aaattacaca atatgaaaag tcactatatt atgccagttt tttggaagtc ttagttcgag
      421 atgtgtgtat ttcattggaa attgatgact tgaaaaaaat taccaattca ctgactgtgc
      481 tttgcagtga aaaacagaag caagaaaagc aaagcaaagc caaatgatga cgaagcttgc
      541 ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa
      601 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
      661 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat tggcgaatgg
      721 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
      781 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      841 acgttcgccg gctttccccg tcaagctct
//
