LOCUS       sequence-7937-ex       10331 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10331)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 10331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG425GPD-ccdB-TAP
FEATURES             Location/Qualifiers
     source          1..10331
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      69..1411
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    401..448
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        1438..1542
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1543..1611,1612..2403)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1761..1780)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2574..3162
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3063..3082
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3316..3333
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(3486..3491,3492..3509,3510..3516)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3524..3540
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3529..3551
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3548..3564
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3548..3564
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3583..3603
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3585..3603
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3632..4276
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     4170..4193
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     primer_bind     4282..4298
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4282..4298
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    4306..4430
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4455..4460,4461..4478,4479..4485)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4539..5198
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4605..4624)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5543..5848
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5727..5746
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5889..6013)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             6072..6149
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             6177..6197
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      6948..7195
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(6964..6982)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(7213..7232)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7214..7232)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7242..7259)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7242..7258)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7251..7273)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7403..7858
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7490..7509)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7700..7721
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8158..8562
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             8575..9669
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVAGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANLLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(10162..10181)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10281..10303
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagtatga tccaatatca aaggaaatga tagcattgaa ggatgagact aatccaattg
      121 aggagtggca gcatatagaa cagctaaagg gtagtgctga aggaagcata cgataccccg
      181 catggaatgg gataatatca caggaggtac tagactacct ttcatcctac ataaatagac
      241 gcatataagt acgcatttaa gcataaacac gcactatgcc gttcttctca tgtatatata
      301 tatacaggca acacgcagat ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc
      361 gcgttgcatt ttcggaagcg ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt
      421 cctattctct agaaagtata ggaacttcag agcgcttttg aaaaccaaaa gcgctctgaa
      481 gacgcacttt caaaaaacca aaaacgcacc ggactgtaac gagctactaa aatattgcga
      541 ataccgcttc cacaaacatt gctcaaaagt atctctttgc tatatatctc tgtgctatat
      601 ccctatataa cctacccatc cacctttcgc tccttgaact tgcatctaaa ctcgacctct
      661 acatttttta tgtttatctc tagtattact ctttagacaa aaaaattgta gtaagaacta
      721 ttcatagagt gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg tagtatatag
      781 agacaaaata gaagaaaccg ttcataattt tctgaccaat gaagaatcat caacgctatc
      841 actttctgtt cacaaagtat gcgcaatcca catcggtata gaatataatc ggggatgcct
      901 ttatcttgaa aaaatgcacc cgcagcttcg ctagtaatca gtaaacgcgg gaagtggagt
      961 caggcttttt ttatggaaga gaaaatagac accaaagtag ccttcttcta accttaacgg
     1021 acctacagtg caaaaagtta tcaagagact gcattataga gcgcacaaag gagaaaaaaa
     1081 gtaatctaag atgctttgtt agaaaaatag cgctctcggg atgcattttt gtagaacaaa
     1141 aaagaagtat agattctttg ttggtaaaat agcgctctcg cgttgcattt ctgttctgta
     1201 aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgtttt
     1261 acaaaaatga agcacagatt cttcgttggt aaaatagcgc tttcgcgttg catttctgtt
     1321 ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt
     1381 gttctacaaa atgaagcaca gatgcttcgt tcaggtggca cttttcgggg aaatgtgcgc
     1441 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1501 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     1561 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     1621 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     1681 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     1741 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     1801 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     1861 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     1921 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     1981 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     2041 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     2101 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     2161 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     2221 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     2281 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     2341 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     2401 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     2461 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     2521 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2581 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2641 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2701 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     2761 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2821 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2881 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2941 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3001 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3061 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3121 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3181 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3241 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3301 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3361 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3421 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt acctcactca ttaggcaccc
     3481 caggctttac actttatgct tccggctcct atgttgtgtg gaattgtgag cggataacaa
     3541 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     3601 agggaacaaa agctggagct cttcgagttt atcattatca atactcgcca tttcaaagaa
     3661 tacgtaaata attaatagta gtgattttcc taactttatt tagtcaaaaa attagccttt
     3721 taattctgct gtaacccgta catgcccaaa atagggggcg ggttacacag aatatataac
     3781 accgaaggtg cctgggtgaa caggtttatt cctggcatcc actaaatata atggagcccg
     3841 ctttttaagc tggcatccag aaaaaaaaag aatcccagca ccaaaatatt gttttcttca
     3901 ccaaccatca gttcataggt ccattctctt agcgcaacta cagagaacag gggcacaaac
     3961 aggcaaaaaa cgggcacaac ctcaatggag tgatgcaacc tgcctggagt aaatgatgac
     4021 acaaggcaat tgacccacgc atgtatctat ctcattttct tacaccttct attaccttct
     4081 gctctctctg atttggaaaa agctgaaaaa aaaggttgaa accagttccc tgaaattatt
     4141 cccctacttg actaataagt atataaagac ggtaggtatt gattgtaatt ctgtaaatct
     4201 atttcttaaa cttcttaaat tctactttta tagttagtct tttttttagt tttaaaacac
     4261 cagaacttag tttcgacgga ttctagaact agtggatccc ccatcacaag tttgtacaaa
     4321 aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat
     4381 aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg gcggccgcat
     4441 taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg attttgagtt
     4501 aggatccgtc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
     4561 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
     4621 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
     4681 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
     4741 atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg
     4801 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
     4861 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
     4921 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
     4981 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     5041 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     5101 cgattcaggt tcatcatgcc gtctgtgatg gcttccatgt cggcagaatg cttaatgaat
     5161 tacaacagta ctgcgatgag tggcagggcg gggcgtaaac gccgcgtgga tccggcttac
     5221 taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata
     5281 ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt
     5341 gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt
     5401 ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg
     5461 gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct
     5521 gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg
     5581 ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt
     5641 gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt
     5701 ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt
     5761 ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc
     5821 cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc
     5881 aggtcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat
     5941 gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct
     6001 tgtacaaagt ggtgatgggc tgcaggaatt cgatatcaag cttatcgata ggtggaccag
     6061 gtggtggaat gaagcgacga tggaaaaaga atttcatagc cgtctcagca gccaaccgct
     6121 ttaagaaaat ctcatcctcc ggggcacttg attatggtac cccaactact gcttctgaaa
     6181 atctatattt tcaaggtgaa ctaaaaactg ctgctttggc tcaacatgcg attaagggtg
     6241 aagctcaaaa acttaatgac tctcaagctc caaaagctga tgcgcaacaa aataacttca
     6301 acaaagatca acaaagcgcc ttctatgaaa tcttgaacat gcctaactta aacgaagcgc
     6361 aacgtaacgg cttcattcaa agtcttaaag acgacccaag ccaaagcact aacgttttag
     6421 gtgaagctaa aaaattaaac gaatctcaag caccgaaagc tgataacaat ttcaacaaag
     6481 aacaacaaaa tgctttctat gaaatcttga atatgcctaa cttaaacgaa gaacaacgca
     6541 atggtttcat ccaaagctta aaagatgacc caagccaaag tgctaaccta ttgtcagaag
     6601 ctaaaaagtt aaatgaatct caagcaccga aagcggataa caaattcaac aaagaacaac
     6661 aaaatgcttt ctatgaaatc ttacatttac ctaacttaaa cgaagaacaa cgcaatggtt
     6721 tcatccaaag cctaaaagat gacccaagcc aaagcgctaa ccttttagca gaagctaaaa
     6781 agctaaatga tgctcaagca ccaaaagctg acaacaaatt caacaaagaa caacaaaatg
     6841 ctttctatga aattttacat ttacctaact taactgaaga acaacgtaac ggcttcatcc
     6901 aaagccttaa agacgatcct tcggtgagca aagaacagtc actcgagtca tgtaattagt
     6961 tatgtcacgc ttacattcac gccctccccc cacatccgct ctaaccgaaa aggaaggagt
     7021 tagacaacct gaagtctagg tccctattta tttttttata gttatgttag tattaagaac
     7081 gttatttata tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg catgtaacat
     7141 tatactgaaa accttgcttg agaaggtttt gggacgctcg aaggctttaa tttgcggccg
     7201 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     7261 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     7321 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     7381 agcctgaatg gcgaatggcg cgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     7441 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     7501 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     7561 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     7621 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     7681 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     7741 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     7801 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttcc
     7861 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tcgacggtcg
     7921 aggagaactt ctagtatatc cacataccta atattattgc cttattaaaa atggaatccc
     7981 aacaattaca tcaaaatcca cattctcttc aaaatcaatt gtcctgtact tccttgttca
     8041 tgtgtgttca aaaacgttat atttatagga taattatact ctatttctca acaagtaatt
     8101 ggttgtttgg ccgagcggtc taaggcgcct gattcaagaa atatcttgac cgcagttaac
     8161 tgtgggaata ctcaggtatc gtaagatgca agagttcgaa tctcttagca accattattt
     8221 ttttcctcaa cataacgaga acacacaggg gcgctatcgc acagaatcaa attcgatgac
     8281 tggaaatttt ttgttaattt cagaggtcgc ctgacgcata tacctttttc aactgaaaaa
     8341 ttgggagaaa aaggaaaggt gagaggccgg aaccggcttt tcatatagaa tagagaagcg
     8401 ttcatgacta aatgcttgca tcacaatact tgaagttgac aatattattt aaggacctat
     8461 tgttttttcc aataggtggt tagcaatcgt cttactttct aacttttctt accttttaca
     8521 tttcagcaat atatatatat atttcaagga tataccattc taatgtctgc ccctatgtct
     8581 gcccctaaga agatcgtcgt tttgccaggt gaccacgttg gtcaagaaat cacagccgaa
     8641 gccattaagg ttcttaaagc tatttctgat gttcgttcca atgtcaagtt cgatttcgaa
     8701 aatcatttaa ttggtggtgc tgctatcgat gctacaggtg tcccacttcc agatgaggcg
     8761 ctggaagcct ccaagaaggt tgatgccgtt ttgttaggtg ctgtggctgg tcctaaatgg
     8821 ggtaccggta gtgttagacc tgaacaaggt ttactaaaaa tccgtaaaga acttcaattg
     8881 tacgccaact taagaccatg taactttgca tccgactctc ttttagactt atctccaatc
     8941 aagccacaat ttgctaaagg tactgacttc gttgttgtca gagaattagt gggaggtatt
     9001 tactttggta agagaaagga agacgatggt gatggtgtcg cttgggatag tgaacaatac
     9061 accgttccag aagtgcaaag aatcacaaga atggccgctt tcatggccct acaacatgag
     9121 ccaccattgc ctatttggtc cttggataaa gctaatcttt tggcctcttc aagattatgg
     9181 agaaaaactg tggaggaaac catcaagaac gaattcccta cattgaaggt tcaacatcaa
     9241 ttgattgatt ctgccgccat gatcctagtt aagaacccaa cccacctaaa tggtattata
     9301 atcaccagca acatgtttgg tgatatcatc tccgatgaag cctccgttat cccaggttcc
     9361 ttgggtttgt tgccatctgc gtccttggcc tctttgccag acaagaacac cgcatttggt
     9421 ttgtacgaac catgccacgg ttctgctcca gatttgccaa agaataaggt tgaccctatc
     9481 gccactatct tgtctgctgc aatgatgttg aaattgtcat tgaacttgcc tgaagaaggt
     9541 aaggccattg aagatgcagt taaaaaggtt ttggatgcag gtatcagaac tggtgattta
     9601 ggtggttcca acagtaccac cgaagtcggt gatgctgtcg ccgaagaagt taagaaaatc
     9661 cttgcttaaa aagattctct ttttttatga tatttgtaca taaactttat aaatgaaatt
     9721 cataatagaa acgacacgaa attacaaaat ggaatatgtt catagggtag acgaaactat
     9781 atacgcaatc tacatacatt tatcaagaag gagaaaaagg aggatagtaa aggaatacag
     9841 gtaagcaaat tgatactaat ggctcaacgt gataaggaaa aagaattgca ctttaacatt
     9901 aatattgaca aggaggaggg caccacacaa aaagttaggt gtaacagaaa atcatgaaac
     9961 tacgattcct aatttgatat tggaggattt tctctaaaaa aaaaaaaata caacaaataa
    10021 aaaacactca atgacctgac catttgatgg agtttaagtc aataccttct tgaagcattt
    10081 cccataatgg tgaaagttcc ctcaagaatt ttactctgtc agaaacggcc ttacgacgta
    10141 gtcgatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
    10201 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
    10261 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
    10321 gaaacgcgcg a
//
