LOCUS       sequence-79375-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 3D8H
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             866..883
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtt ccgcgtggta gtacttacaa gctaacattg attcgtcatg
      121 gtgaaagtga gtggaacaaa gaaaataggt ttactggttg gacagatgtt agcctcagtg
      181 agcaaggagt aagcgaggca attgaggcag gaagaatgct tttagagaaa ggcttcaaat
      241 tcgatgtagt ttacacttca gtattaaaga gggctattat gactacatgg actgtcttaa
      301 aggaactagg taatattaat tgcccaatta ttaaccactg gagattaaac gaaagacatt
      361 acggagcttt gcaagggctc aataagtctg aaactgcctc caagtttgga gaggaccaag
      421 tcaagatttg gagaagaagt tttgatgttc ctcctccagt tcttgaaaag tctgatccaa
      481 gatggccagg aaatgagttg atctataagg gtatctgtcc ctcatgctta ccaacaacag
      541 aatgtttgaa ggataccgtt gaaagagtta agccgtattt cgaggacgta attgctcctt
      601 caatcatgtc aggaaaatct gtcctagttt ctgctcatgg gaattccctt agagctcttc
      661 tctacttgct agaaggaatg acaccagaac aaattcttga ggttaatatt cccacagcat
      721 gtccacttgt attggagctt gacgattatc tcaaggtgac aaagaaatac tatttgatta
      781 gcgaggaaga actcaaggct aagatggagg ctgttgcgaa tcaaggaaaa gctaagtgat
      841 gacgaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa
//
