LOCUS       sequence-79378-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 3S8P
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatgca tcatcatcat catcacagca
       61 gcggcagaga aaacttgtat ttccagggcc agagtcgcta tgtaccatcc tctggaatgt
      121 ccgccaagga actctgtgaa aatgatgacc tagcaaccag tttggttctt gatccctatt
      181 taggttttca aacacacaaa atgaatacta gcgcctttcc ttcgaggagc tcaaggcatt
      241 tttcaaaatc tgacagtttt tctcacaaca accctgtgag atttaggcct attaaaggaa
      301 ggcaggaaga actaaaggaa gtaattgaac gttttaagaa agatgaacac ttggagaaag
      361 ccttcaaatg tttgacttca ggcgaatggg cacggcacta ttttctcaac aagaataaaa
      421 tgcaggagaa attattcaaa gaacatgtat ttatttattt gcgaatgttt gcaactgaca
      481 gtggatttga aatattgcca tgtaatagat actcatcaga acaaaatgga gccaaaatag
      541 ttgcaacaaa agagtggaaa cgaaatgaca aaatagaatt actggtgggt tgtattgccg
      601 aactttcaga aattgaggag aacatgctac ttagacatgg agaaaacgac ttcagtgtca
      661 tgtactccac aaggaaaaac tgtgctcaac tctggctggg tcctgctgcg tttataaacc
      721 atgattgcag acctaattgt aagtttgtgt caactggtcg agatacagca tgtgtgaagg
      781 ctctaagaga cattgaacct ggagaagaaa tttcttgtta ttatggagat gggttctttg
      841 gagaaaataa tgagttctgc gagtgttaca cttgcgaaag acggggcact ggtgctttta
      901 aatccagatg atgacgaagc ttgcg
//
