LOCUS       sequence-79383-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 3FK2
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             803..820
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(873..891)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatgca tcatcatcat catcacagca
       61 gcggcagaga aaacttgtat ttccagggcg acccgcgccg tcgcaatatt ctgcgtagtc
      121 tgcgccgtaa cacaaaaaaa ccgaagccga aaccgcgccc aagcatcact aaagcaacgt
      181 gggaatctaa ttattttggg gtcccgctga ccacagttgt gactccggag aagccgattc
      241 cgatcttcat tgaacgttgc atcgagtaca ttgaagccac gggcctgtcg accgaaggta
      301 tctatcgcgt atcaggcaac aaaagtgaga tggaaagcct gcagcgtcaa tttgatcagg
      361 atcataatct ggacctggcg gagaaagatt tcacagtcaa cactgttgca ggtgcgatga
      421 agtccttttt ctcggaactg ccggacccgc tggtgccgta caatatgcag attgatctgg
      481 tagaagctca taaaatcaac gaccgcgagc aaaaactgca cgccctgaag gaagtcctga
      541 aaaaatttcc gaaggaaaat catgaggttt ttaaatatgt gatttcacac ctgaacaaag
      601 tttcccataa taacaaggtg aatctgatga cgtctgaaaa cctgagtatt tgtttctggc
      661 cgaccctgat gcgtccggat tttagcacca tggatgcgct gacagctact cgcacgtacc
      721 agaccatcat tgagctgttc atccaacagt gcccgttttt cttttataat cgttgatgac
      781 gaagcttgcg gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa
      841 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
      901 tggggcctct aaacgggtct tg
//
