LOCUS       sequence-79384-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 2I62
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             363..374
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatgca tcatcatcat catcacagca
       61 gcggcagaga aaacttgtat ttccagggca tggaatctgg cttcacctcc aaggacactt
      121 atctaagtca ttttaatcct cgggattact tggaaaaata ttacagcttt gggtccagac
      181 actgtgcaga aaatgagatc ctcagacatc tgctgaaaaa tctttttaag atattctgcc
      241 tgggtgctgt aaagggagaa ctcctgattg acattggctc tggccccacc atctatcagc
      301 ttctctctgc ctgtgagtcc ttcacggaga tcattgtctc tgactacaca gaccagaacc
      361 tctgggagct gcagaaatgg ctgaagaagg aaccaggagc ctttgactgg tccccagtgg
      421 tcacctatgt gtgtgatctt gaaggcaaca gaatgaaggg acctgagaag gaggagaagt
      481 tgaggcgtgc aatcaagcag gtactaaagt gtgatgtgac ccagagccag cctctgggtg
      541 gggtctctct gcctcctgct gactgcctgc tcagcacact gtgcctggat gctgcctgcc
      601 ctgacctccc agcctatcgc actgccctca ggaacctggg cagtctgctc aagccaggag
      661 gcttcctggt gatggtagat gccctgaaga gtagctacta catgattggg gagcagaagt
      721 tttccagcct tcccctggga tgggagactg ttcgagatgc tgtggaagag gctggttaca
      781 ccattgagca atttgaggtg atttctcaaa attactcttc taccacatcc aataatgaag
      841 gactcttctc cctagtgggg cgaaagccag gcagatctga atgatgacga a
//
