LOCUS       sequence-79387-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 2LVA
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             452..469
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(522..540)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      536..583
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(707..726)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatgca tcatcatcat catcacagca
       61 gcggcagaga aaacttgtat ttccagggcc aaatgctgtt aaatcaactg agagaaatca
      121 caggcattca ggacccttcc tttctccatg aagctctgaa ggccagtaat ggtgacatta
      181 ctcaggcagt cagccttctc actgatgaga gagttaagga gcccagtcaa gacactgttg
      241 ctacagaacc atctgaagta gaggggagtg ctgccaacaa ggaagtatta gcaaaagtta
      301 tagaccttac tcatgataac aaagatgatc ttcaggctgc cattgctttg agtctactgg
      361 agtctcccaa aattcaagct gatggaagag atcttaacag gatgcatgaa gcaacctctg
      421 catgatgacg aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc
      481 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
      541 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
      601 atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
      661 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
      721 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
      781 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
      841 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
      901 tccacgttct ttaatagtgg
//
