LOCUS       sequence-79390-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4H4H
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..33
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             43..60
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 accatgggca gcagccatca tcatcatcat cacagcagcg gcctggttcc gcgtggtagt
       61 aactctcagg tgtggagcgc tctgcagatg agcaaggctc tgcaggactc ggcccccgac
      121 aggcccagcc ccgtggagag gatagtgagc agggacatcg ctcgaggcta cgagcgcatc
      181 cccatcccct gtgtcaacgc cgtggacagc gagccatgcc ccagcaacta caagtacgtc
      241 tctcagaact gcgtgacgtc ccccatgaac atcgacagaa atatcactca tctgcagtac
      301 tgcgtgtgca tcgacgactg ctcctccagc aactgcatgt gcggccagct cagcatgcgc
      361 tgctggtacg acaaggatgg ccggctcctg ccagagttca acatggcgga gcctcccttg
      421 atcttcgaat gcaaccacgc gtgctcctgc tggaggaact gccgaaatcg cgtcgtacag
      481 aatggtctca gggcaaggct gcagctctac cggacgcggg acatgggctg gggcgtgcgg
      541 tccctgcagg acatcccacc aggcaccttt gtctgcgagt atgttgggga gctgatttca
      601 gactcagaag ccgacgttcg agaggaagat tcttacctct ttgatctcga caataaggac
      661 ggggaggttt actgcatcga cgcgcggttc tacgggaacg tcagccggtt catcaaccac
      721 cactgcgagc ccaacctggt gcccgtgcgc gtgttcatgg cccaccagga cctgcggttc
      781 ccccggatcg ccttcttcag cacccgcctg atcgaggccg gcgagcagct cgggtttgac
      841 tatggagagc gcttctgg
//
