LOCUS       sequence-79391-e         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 2P5S
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             662..679
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(732..750)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      746..794
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(918..937)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtt ccgcgtggta gtgctaaatc ttttagctca cagaaggctt
      121 acaagattgt acttgctggg gacgctgcag tggggaagtc tagtttcctc atgagacttt
      181 gcaagaatga atttcgagaa aatataagcg ccaccctggg agttgatttc caaatgaaaa
      241 ccctcattgt ggatggagaa cgaacagttc tgcagctctg ggatacagct ggtcaggaga
      301 gattcagaag tattgccaag tcttacttca gaaaggcaga tggtgttttg ctgctgtatg
      361 atgttacatg tgagaaaagc tttcttaaca tacgagaatg ggtagatatg attgaggatg
      421 cagcccatga gactgttccc attatgctgg taggaaacaa ggctgacatt cgtgacactg
      481 ctgctacaga gggacaaaaa tgtgtcccag ggcactttgg agagaaactg gccatgacgt
      541 atggggcatt attctgtgaa acaagtgcca aagatggttc taacatagtg gaggctgttc
      601 tgcaccttgc tcgagaagtg aaaaagagaa cttgatgacg aagcttgcgg ccgcactcga
      661 gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt
      721 ggctgctgcc accgctgagc aataactagc ataaccccct tggggcctct aaacgggtct
      781 tgaggggttt tttgctgaaa ggaggaacta tatccggatt ggcgaatggg acgcgccctg
      841 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
      901 cagcgcccta gcgcccgctc ctttcgcttt cttccctt
//
