LOCUS       sequence-79392-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 2NQ3
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             584..601
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(654..672)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      668..715
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(839..858)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatgca tcatcatcat catcacagca
       61 gcggcagaga aaacttgtat ttccagggca tgtctgacag tggatcacaa cttggttcaa
      121 tgggtagcct caccatgaaa tcacagcttc agatcactgt catctcagca aaacttaagg
      181 aaaataagaa gaattggttt ggaccaagtc cttacgtaga ggtcacagta gatggacagt
      241 caaagaagac agaaaaatgc aacaacacaa acagtcccaa gtggaagcaa ccccttacag
      301 ttatcgttac ccctgtgagt aaattacatt ttcgtgtgtg gagtcaccag acactgaaat
      361 ctgatgtttt gttgggaact gctgcattag atatttatga aacattaaag tcaaacaata
      421 tgaaacttga agaagtagtt gtgactttgc agcttggagg tgacaaagag ccaacagaga
      481 caataggaga cttgtcaatt tgtcttgatg ggctacagtt agagtctgaa gttgttacca
      541 atggtgaaac tacatgatga cgaagcttgc ggccgcactc gagcaccacc accaccacca
      601 ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
      661 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
      721 aggaggaact atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
      781 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
      841 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
      901 aatcgggggc tcccttt
//
