LOCUS       sequence-79393-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 2P0K
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatgca tcatcatcat catcacagca
       61 gcggcagaga aaacttgtat ttccagggca tgctggtttg ctacagtgtt ttagcttgtg
      121 agattctctg ggaccttccc tgctccatca tggggtcacc tctaggtcat tttacctggg
      181 acaaatacct aaaagaaaca tgttcagtcc cagcgcctgt ccattgcttc aagcagtcct
      241 acacacctcc aagcaacgag ttcaagatca gtatgaaatt ggaagcacag gaccccagga
      301 acaccacatc cacctgtatt gccacagtag ttggactgac aggtgcccgc cttcgcctgc
      361 gccttgatgg gagcgacaac aaaaatgact tctggcggct ggttgactca gctgaaatcc
      421 agcctattgg gaactgtgaa aagaatgggg gtatgctaca gccacctctt ggatttcggc
      481 tgaatgcgtc ttcttggccc atgttccttt tgaagacgct aaatggagca gagatggctc
      541 ccatcaggat tttccacaag gagccaccat cgccttccca caacttcttc aaaatgggaa
      601 tgaagctaga agctgtggac aggaagaacc ctcatttcat ttgcccagcc actattgggg
      661 aggttcgggg ctcagaggtg cttgtcactt ttgatgggtg gcgaggggcc tttgactact
      721 ggtgccgctt cgactcccga gacatcttcc ctgtgggctg gtgttccttg actggagaca
      781 acctgcagcc tcctggcacc aaagttgtga ttccaaagaa tccctatcct gcctccgatt
      841 gatgacgaa
//
