LOCUS       sequence-79397-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4IA9
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatgca tcatcatcat catcacagca
       61 gcggcagaga aaacttgtat ttccagggca ctcagagcaa gcctacacct gtgaagccaa
      121 actatgctct aaagttcacc cttgctggcc acaccaaagc agtgtcctcc gtgaaattca
      181 gcccgaatgg agagtggctg gcaagttcat ctgctgataa acttattaaa atttggggcg
      241 cgtatgatgg gaaatttgag aaaaccatat ctggtcacaa gctgggaata tccgatgtag
      301 cctggtcgtc agattctaac cttcttgttt ctgcctcaga tgacaaaacc ttgaagatat
      361 gggacgtgag ctcgggcaag tgtctgaaaa ccctgaaggg acacagtaat tatgtctttt
      421 gctgcaactt caatccccag tccaacctta ttgtctcagg atcctttgac gaaagcgtga
      481 ggatatggga tgtgaaaaca gggaagtgcc tcaagacttt gccagctcac tcggatccag
      541 tctcggccgt tcattttaat cgtgatggat ccttgatagt ttcaagtagc tatgatggtc
      601 tctgtcgcat ctgggacacc gcctcgggcc agtgcctgaa gacgctcatc gatgacgaca
      661 acccccccgt gtcttttgtg aagttctccc cgaacggcaa atacatcctg gccgccacgc
      721 tggacaacac tctgaagctc tgggactaca gcaaggggaa gtgcctgaag acgtacactg
      781 gccacaagaa tgagaaatac tgcatatttg ccaatttctc tgttactgg
//
