LOCUS       sequence-79402-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 3L43
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatgca tcatcatcat catcacagca
       61 gcggcagaga aaacttgtat ttccagggca tggaggagct gatcccgctg gtgaaccgtc
      121 tgcaggacgc gttttcggcg ctgggacaga gctgcctgct ggagctgccg cagatcgccg
      181 tggtgggcgg ccagagcgcc ggcaagagct cggtgctcga gaacttcgtg ggcagggact
      241 ttctccctcg agggtcgggc attgtaacaa gacgacctct tgtgctgcag cttattactt
      301 ctaaagcaga atatgccgag tttctacatt gcaaaggaaa gaaatttaca gattttgatg
      361 aagttcgcct tgagattgaa gcagaaacag atcgcgtgac tggaatgaat aaaggcattt
      421 cctccatacc cattaattta cgagtctatt ccccacacgt gttaaatcta acccttattg
      481 atctacctgg aataactaaa gtgcctgtgg gagatcagcc accagatatc gagtatcaga
      541 tcagagaaat gattatgcag ttcatcacga gggagaactg tctgatttta gctgttactc
      601 cagccaacac tgatcttgca aactcagatg cgctgaagct agctaaagaa gttgatcctc
      661 aaggtctgag aaccattgga gttatcacca aactggacct tatggatgaa ggaacggatg
      721 ccagggatgt tctagagaac aaactgttgc ctcttcgcag gggttacgtg ggggtggtaa
      781 acagaagcca gaaggacata gatgggaaga aggacataaa ggcagccatg ctggcagaga
      841 ggaagttttt cctttcccac ccggcttaca gacatatcgc tgaccgaatg gg
//
