LOCUS       sequence-79404-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 3CKL
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtt ccgcgtggta gtatgctttc cccaaaagat attctgcgaa
      121 aagatctgaa gttggtccat ggttatccca tgacctgtgc ttttgcaagc aactgggaaa
      181 aaattgaaca gttccatagc agaccagatg acattgtgat agccacttat cctaaatcag
      241 gtactacttg ggttagtgaa attatagaca tgattctaaa tgatggagat attgaaaaat
      301 gtaagcgagg ttttattact gaaaaagttc caatgttgga aatgactctc cctggattaa
      361 gaacatcagg tatagaacaa ttggagaaga atccatcacc ccggattgtg aaaacacatc
      421 taccgactga tcttcttcct aaatctttct gggaaaacaa ttgcaagatg atttatctgg
      481 ctcgtaatgc caaggatgtt tcagtctcat attaccattt tgacttaatg aataatttac
      541 agccttttcc tggtacctgg gaagaatatc tggagaaatt cttaactgga aaagtggcct
      601 atggttcctg gtttactcat gttaaaaact ggtggaagaa gaaggaagaa cacccaatac
      661 tttttttgta ctatgaagat atgaaagaga atccaaagga ggaaatcaag aagatcatta
      721 gatttctaga gaagaacctg aatgatgaga tcttggatag gatcatccat cacacctcat
      781 ttgaagtgat gaaggacaat cctttggtaa attatacaca tctaccaact acagtgatgg
      841 atcatagcaa atcccctttt atgcgtaaag ggacggctgg tgact
//
