LOCUS       sequence-79405-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 2PSW
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             626..643
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(696..714)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      710..757
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(881..900)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtt ccgcgtggta gtaaaggtag ccggatcgag ctgggagatg
      121 tgacaccaca caatattaaa cagttgaaaa gattgaatca ggtcatcttt ccagtcagct
      181 acaatgacaa gttctacaag gatgtgctgg aggttggcga gctagcaaaa cttgcctatt
      241 tcaatgatat tgctgtaggt gcagtatgct gtagggtgga tcattcacag aatcagaaga
      301 gactttacat catgacacta ggatgtctgg caccttaccg aaggctagga ataggaacta
      361 aaatgttaaa tcatgtctta aacatctgtg aaaaagatgg tacttttgac aacatttatc
      421 tgcatgtcca gatcagcaat gagtcggcaa ttgacttcta caggaagttt ggctttgaga
      481 ttattgagac aaagaagaac tactataaga ggatagagcc cgcagatgct catgtgctgc
      541 agaaaaacct caaagttcct tctggtcaga atgcagatgt gcaaaagaca gacaactgat
      601 gacgaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa
      661 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
      721 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg
      781 attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
      841 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
      901 tcctttctcg ccacgttcgc cggctttccc
//
