LOCUS       sequence-79414-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 2GSF
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtt ccgcgtggta gtagccaagt ggtcatgatc gccatttcag
      121 cggcagtagc aattattctc ctcactgttg tcatctatgt tttgattggg aggttctgtg
      181 gctataagtc aaaacatggg gcagatgaaa aaagacttca ttttggcaat gggcatttaa
      241 aacttccagg tctcaggact tatgttgacc cacatacata tgaagaccct acccaaactg
      301 ttcatgagtt tgccaaggaa ttggatgcca ccaacatatc cattgataaa gttgttggag
      361 caggtgaatt tggagaggtg tgcagtggtc gcttaaaact tccttcaaaa aaagagattt
      421 cagtggccat taagaccctg aaagttggct acacagaaaa gcagaggaga gacttcctgg
      481 gagaagcaag cattatggga cagtttgacc accccaatat cattcgactg gaaggagttg
      541 ttaccaaaag taagccagtt atgattgtca cagaatacat ggagaatggt tccttggata
      601 gtttcctacg taaacacgat gcccagttta ctgtcattca gctagtgggg atgcttcgag
      661 ggatagcatc tggcatgaag tacctgtcag acatgggcta tgttcaccga gacctcgctg
      721 ctcggaacat cttgatcaac agtaacttgg tgtgtaaggt ttctgatttc ggactttcgc
      781 gtgtcctgga ggatgaccca gaagctgctt atacaacaag aggagggaag atcccaatca
      841 ggtggacatc accagaagct atagcctacc gcaagttcac gtcagccagc gatgtatgga
      901 gttatgggat tg
//
