LOCUS       sequence-79415-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 2NQA
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtt ccgcgtggta gtaacgcttt gaagtacttg ggccaggatt
      121 tcaagaccct gaggcaacag tgcttggact caggggtcct atttaaggac cctgagttcc
      181 cagcatgtcc atcagctttg ggctacaagg atcttggacc aggctctccg caaactcaag
      241 gcatcatctg gaagcggccc acggagttgt gtcccagccc tcagtttatc gttggtggag
      301 ccacgcgcac agacatttgt cagggtggtc taggtgactg ctggcttctg gctgccattg
      361 cctccctgac cctgaatgaa gagctgcttt accgggtggt ccccagggac caggacttcc
      421 aggagaacta tgcgggaatc tttcactttc agttctggca gtacggagag tgggtggagg
      481 tggtcattga cgacaggctg cccaccaaga atggacagct gctcttccta cactcggaac
      541 aaggcaatga attctggagt gccctgctgg agaaagccta tgccaagctt aatggttgtt
      601 atgaggctct cgctggaggt tccacagtgg aggggtttga ggatttcaca ggtggcatct
      661 ctgagtttta tgacctgaag aaaccaccag ccaatctata tcagatcatc cggaaggccc
      721 tctgtgcggg gtctctgctg ggctgctcca ttgatgtcta cagtgcagcc gaagccgaag
      781 ccatcaccag ccagaagctg gttaagagtc atgcgtactc tgtcactgg
//
