LOCUS       sequence-79416-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4HAE
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             308..325
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(378..396)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      392..439
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      476..904
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(563..582)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     773..794
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatgca tcatcatcat catcacagca
       61 gcggcagaga aaacttgtat ttccagggcg cttctgggga cctttacgag gttgaaagga
      121 ttgtagacaa gaggaagaac aagaaaggaa aatgggagta tcttatccga tggaaaggct
      181 acgggagcac cgaggacacg tgggagccgg agcaccacct cttgcactgt gaggagttta
      241 ttgatgaatt caatgggttg cacatgtcca aggacaagtg atgacgaagc ttgcggccgc
      301 actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc
      361 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
      421 ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga atgggacgcg
      481 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
      541 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
      601 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
      661 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
      721 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
      781 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
      841 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
      901 aattttaaca aaatattaac gctta
//
