LOCUS       sequence-79417-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  TITLE     SGC Plasmids - 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 2I81
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             699..716
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(769..787)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      783..830
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggttccgcg tggtagtccg acatacgtag gaaaggaagc cccctttttc
      121 aaagctgagg ccgtttttgg agataactcc tttggagaag tgaatttaac acaatttatc
      181 ggaaagaagt atgtgctgtt atatttttac cctcttgatt ttaccttcgt gtgcccatca
      241 gaaataatag cattggataa agccttggat gcatttcacg aaaggaatgt agaattgttg
      301 ggatgcagtg tagatagcaa atacacacac cttgcatgga aaaaaacccc actggcaaaa
      361 ggaggcattg gcaatatcaa gcatacttta ctatctgaca ttacgaaaag catttcaaag
      421 gactacaatg ttctctttga tgatagcgta tctttgaggg ccttcgtttt gatcgacatg
      481 aatggaattg tgcaacatct tctcgttaac aatctggcaa taggtcgctc agtggacgaa
      541 attttacgaa taatcgacgc catacagcac catgagaaat acggcgatgt gtgtccagcc
      601 aactggcaaa agggcaaggt gtccatgaag ccatcagagg agggcgttgc gcagtatttg
      661 tccacattat gatgacgaag cttgcggccg cactcgagca ccaccaccac caccactgag
      721 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
      781 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
      841 gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa
//
